16S V2-V3 Library Preparation Kit for Illumina

The 16S V2-V3 Library Preparation Kit for Illumina consists of the reagents andcomponents required for library preparation of the 16S V2-V3 amplicon librariesto be used for next-generation sequencing on Illumina platforms. All molecularreagents including primers, enzyme mixes, indexes, and buffers are provided.Instructions for PCR clean up with the AMPure XP Magnetic Beads (supplied bycustomer) are also included for rapid purification of nucleic acid productsgenerated at two steps of the workflow. The library prep workflow could be usedfor purified DNA inputs from different sources including stool, soil, water, saliva,plant, urine, skin swab, vaginal swab, cheek swab, nasal swab, plasma/serum,tongue swab, gum swab, and others.The 16S V2-V3 Library Preparation Kit for Illumina has a streamlined procedurethat reduces the handling time such that the library prep procedure can becompleted in approximately 4 hours (see diagram below). Input DNA is firstsubjected to targeted PCR to amplify the V2-V3 region of the DNA encoding 16SrRNA. The post-PCR reaction is then cleaned up using AMPure XP beads. Dualindex primers are then added using a limited-cycle PCR. The indexed ampliconsflanked by 5' and 3' barcoded adaptors are then cleaned using AMPure XPbeads. The libraries are then ready for quantification, pooling and sequencing.