Plum Pox Virus RT-PCR Detection Kit

Norgen’s Plum Pox Virus RT-PCR Detection Kit is a ready-to-use system for the isolation and detection of Plum Pox Virus from plant samples. First, the kit contains components for the rapid isolation of total RNA, including viral RNA, from plant samples using spin-column chromatography based on Norgen’s proprietary resin. Second, the kit contains Plum Pox Virus Master Mix and controls to allow for PCR amplification, as well as a Control Master Mix to allow for amplification of both an isolation control and a PCR control. The amplified PCR products are then detected using agarose gel electrophoresis. Alternatively, detection can be performed based on real-time PCR using melt curves.The Plum Pox Virus Master Mix contains reagents and enzymes for the specific amplification of a 295 bp region of the viral genome. In addition, Norgen’s Plum Pox Virus RT-PCR Detection Kit contains a second Mastermix, the Control 2X RT-PCR Master Mix, which can be used to identify possible PCR inhibition and/or inadequate isolation via a separate RT-PCR reaction with the use of the provided PCR control (PCRC) or Isolation Control (IsoC), respectively. This kit is designed to allow for the testing of 24 samples. The Plum Pox Virus RT-PCR Detection Kit is also available in a 96-well format for high throughput analysis. The Plum Pox Virus RT-PCR Primer Set and Controls Controls are also available separately for end-point PCR detection,