COVID-19 TaqMan RT-PCR Kit (N/ORF1ab genes)

Norgen’s COVID-19 TaqMan RT-PCR Kit (N/ORF1ab genes) includes 2X One-Step RT-PCR Master Mix, a primer/probe mix, a positive control and a negative control (nuclease-free water). The primer/probe mix contains three SARS-CoV-2 detection targets N (Nucleoprotein), ORF1ab (Open reading frame1ab) and the human RNase P transcript (RP - HEX/VIC) as an internal control target to monitor for PCR inhibition, and to validate the quality of the sample and the detection result. The provided N/ORF1ab/RP Positive Control contains an in vitro RNA transcript for the two SARS-related target genes: N gene, ORF1ab gene as well as the human RP gene (internal control).This kit was developed and validated to be used with the following PCR instruments: Qiagen Rotor-Gene Q, BioRad CFX96 Touch™ Real-Time PCR Detection System, ABI 7500, ABI Quantstudio 5 and 7. KeywordsCOVID Coronavirus PCR SARS-CoV-2